Coding
clyA_Nter

Part:BBa_K257007:Design

Designed by: Guillaume Cambray   Group: iGEM09_Paris   (2009-09-10)

ClyA_Fusion_N-Term


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 84
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For fusion of proteins to the N-terminal extremity of ClyA using the silver fusion standard RFC23. We also need a poly gly linker at the N-terminal extremity.

This part is inserted in PSB1A3

Source

Sequence from E. coli k 12 strain MG1655 (refseq accessor NC_000913.2)

References